Mechanism Studies of Suppressor-Gene Action

Mechanism studies of suppressor-gene action.

Brody, Stuart (Stanford University, Stanford, Calif.), and Charles Yanofsky. Mechanism studies of suppressor-gene action. J. Bacteriol. 90:687-695. 1965.-Mutations which change the primary structure of the A protein of the tryptophan synthetase of Escherichia coli can be reversed by allele-specific suppressor mutations. Normally, the suppressors of a particular A mutant lead to the appearance o...

Studies on the Mechanism of Interferon Action

Interferon does not inactivate viruses or viral RNA. Virus growth is inhibited in interferon-treated cells, but apart from conferring resistance to virus growth, no other effect of interferon on cells has been definitely shown to take place. Interferon binds to cells even in the cold, but a period of incubation at 37 degrees C is required for development of antiviral activity. Cytoplasmic uptak...

Loperamide: studies on its mechanism of action.

The effects of loperamide on net solute and water absorption, and prostaglandin E2 (PGE2) and cholera toxin-induced secretion were studied in the rat jejunum using an in vivo steady-state perfusion technique. Loperamide stimulated absorption of fluid, electrolytes, and glucose and reversed PGE2 and cholera toxin-induced secretion to absorption; this opiate analogue had no effect on cholera toxi...

Mechanism of rhodanese action: isotopic tracer studies.

In a previous publication (2) a double displacement mechanism for the catalytic activity of rhodanese (thiosulfate cyanide sulfurtransferase) was proposed on the basis of polarographic evidence. In addition it was concluded from the polarographic data that crystahine beef liver rhodanese is an enzyme-substrate intermediate containing two activated substrate sulfur atoms per molecule. This commu...

Studies on the mechanism of hormone action.